How does ethidium bromide interact with dna

This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. Since DNA molecules are sized by their relative movement through a gel compared to a molecular weight standard, mobility measurements can be critical to size determinations. Received 23 Sep Revised 17 Nov Accepted 19 Nov Published 01 Dec Introduction Noncovalent binding of different compounds ligands with DNA is an object of numerous studies, since these substances possess high biological activity and influence on many vitally important processes occurring in the cells.

Results and Discussion Among biologically active compounds interacting with DNA and significantly influencing its structural-functional characteristics EtBr takes a special place binding to DNA both in vivo and in vitro and inhibiting replication and transcription processes [ 2 — 4 , 6 ].

Figure 1. Concentration of DNA was equal to 7. Figure 2. References S. Neible and M. Lane and T. Vardevanyan, A. Antonyan, M. Parsadanyan, K. Pirumyan, A. Muradyan, and A. Antonyan, G. Manukyan, and A. Wadkins, E. Jares-Erijman, R. Klement, A. Parsadanyan, H. Davtyan, and A. Veselkov, S. Baranovkii, L. Dymant et al. View at: Google Scholar S. Nafisi, A. Saboury, N. Keramat, J. Neault, and H. Hossain, P. Giri, and G.

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Kostyukov V V. Energy of intercalation of aromatic heterocyclic ligands into DNA and its partition into additive components. Biopolym Cell. Ab initio quantum chemical, density functional theory, and empirical potential study. Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Sign In. Advanced Search. Search Menu. Article Navigation.

Close mobile search navigation Article Navigation. Volume Article Contents Abstract. Ethidium bromide interactions with DNA: an exploration of a classic DNA—ligand complex with unbiased molecular dynamics simulations.

Single-stranded oligonucleotides can still have secondary structure when they fold back upon themselves and base pair. EtBr can intercalate between base pairs, whether they are from the same strand or a second strand. Could anyone tell me? I have a question Regarding EtBr. If there are two DNA molecules with same size,one is linear and another one is linear with replication bubble. More Recent Comments. Last Monday's Molecule was ethidium, better known by the name of its common salt, ethidium bromide [ Monday's Molecule 35 ].

Ethidium is a large planer molecule that binds tightly to DNA. It is often used in biochemistry laboratories to visualize fragments of DNA that have been separated on gels. The ethidium molecule is fluorescent—when illuminated with ultraviolet light it shines in the visible range. Here's a picture below right of DNA fragments that are illuminated by ethidium binding.